Plasmid

Part:BBa_K382002:Design

Designed by: Mara C. Inniss   Group: iGEM10_Harvard   (2010-10-26)


pORE Expression Series Vector with BioBrick MCS, Basta resistance


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5326
    Illegal XbaI site found at 5299
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5326
    Illegal NheI site found at 4766
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
    Illegal NotI site found at 5341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5326
    Illegal BglII site found at 5212
    Illegal BglII site found at 7771
    Illegal BamHI site found at 5312
    Illegal XhoI site found at 4790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5326
    Illegal XbaI site found at 5299
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5326
    Illegal XbaI site found at 5299
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 2772
    Illegal NgoMIV site found at 7220
    Illegal AgeI site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7241


Design Notes

Silver standard 21


Source

Modified from pORE_E3 found in Arabidopsis Information Resource

References